Yeast Genetics: A Manual of Methods - John F.T. Spencer - Books - Springer-Verlag Berlin and Heidelberg Gm - 9783540188056 - December 23, 1988
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John F.T. Spencer
Yeast Genetics: A Manual of Methods

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This book is designed for use as a manual of methods, to be used at the bench. Topics discussed and methods described include classical genetics as related to yeasts, such as mating, sporulation, isolation of hybrids, microdissection of asci or the isolation of single-spore clones, mapping of genes and the construction of new strains by protoplast fusion. There is a large section dealing with mutations in general, and with methods of isolating a number of important classes of mutants in particular. A second part deals with basic techniques in separation of chromosomes by electrophoresis, such as OFAGE, FIGE, and CHEF, and gives detailed protocols for the first two. This section further includes methods for isolation of high molecular weight DNA from yeast, isolation of RNA, and techniques for transformation of yeasts.

104 pages, 4 black & white illustrations, biography

Media Books     Paperback Book   (Book with soft cover and glued back)
Released December 23, 1988
ISBN13 9783540188056
Publishers Springer-Verlag Berlin and Heidelberg Gm
Pages 104
Dimensions 155 × 235 × 6 mm   ·   172 g
Language English  
Editor Spencer, John F.t.

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