Debasish Ghosh

Molecular Cell Biology of Potato Yellow Dwarf Virus N and P Protein: Molecular and Cellular Characterization of Pydv Nucleocapsid and Phosphoprotein; Understanding the Biology of Pydv Debasish Ghosh

Potato yellow dwarf virus (PYDV) is the type member of the genus Nucleorhabdovirus. There are two different strains of PYDV based on their insect-vector specificity, namely SYDV (sanguinolenta) and CYDV (constricta). PYDV is considered a model system to study virus-vector relationship for agriculturally harmful rhabdoviruses. This book describes the complete characterization of nucleotide and ORF sequences of N (nucleocapsid) and P (Phosphoprotein) gene of SYDV from cDNA clones of both viral genomic and messenger RNAs. Comparison of the SYDV-N ORF with orthologous regions from other plant and animal rhabdoviruses showed statistically significant identity. Phylogenetic analysis based on consensus N-ORFs placed SYDV into the same group with other Nucleorhabdoviruses. Sub cellular localization studies of SYDV-N and P protein revealed that both proteins are exclusively nuclear localized. Taken together, this book reports a detailed analysis of the biology of SYDV-N and P protein at the molecular and cellular level for the first time towards the long-term goal to characterize the entire SYDV genome and to better understand SYDV-host interaction.