Shami Chakrabarti

Profiling of Quinolone-resistant Plasmids from Pyogenic Skin Infection: Molecular Diagnosis of Plasmid Mediaated Quinolone Resistance Shami Chakrabarti

In this study we have collected 21 patient samples having pyogenic skin infection to assess their antibiotic susceptibility by disc diffusion method and to isolate quinolone-resistant strains. Among 21 patient samples only four samples were quinolone-susceptible. A few of the quinolone-resistant samples are also methicillin resistant. The majority of the clinical isolates were gram-positive.8 samples within the 17 clinical isolates gave positive result in plasmid isolation.1 quinolone-susceptible but methicillin resistant strain also yielded plasmids. These 9 samples also gave distinct band pattern when they were digested with restriction enzyme EcoRI, HindIII, HinfI. Those isolated plasmids were transformed to E. coli DH5? strain,among the 8 samples 6 samples transformed quinolone resistance to sensitive cells.